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EM Protocols

Friday, June 12, 2009

Solutions and Formulas

Buffers
0.1M Cacodylate Buffer containing 4% Sucrose and 0.1M Glycine
0.1M Cacodylate Buffer containing 4% sucrose
White's Buffer Solution
0.18M Phosphate Buffer
0.1M Tris Wash
0.4M Phosphate Buffer Stock
0.1M Phosphate Buffer from Monobasic and Dibasic Stock

Fixatives
3% Paraformaldehyde for Immuno Electron Microscopy (Cacodylate Buffer)

Mouse Gluteraldehyde Fixative
Perfusion Fixative in Phosphate Buffer (1%GA, 4%PFA)
2% GA, 2%PFA in 0.1M Phosphate Buffer
2% GA, 2%PFA in 0.1M Cacodylate Buffer
2.5% GA, 4% PFA in 0.1M CB, 0.1M Sucrose
2% GA in 0.05M PB, 0.1M Sucrose
2% OsO4
1% OsO4

Resins


Stains
Urynal Acetate and Lead Citrate for EM section staining
Tuloidine Blue for Semi-Thin OLM Sections
Posted by Eric W. Roth at 6:44 AM

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Eric W. Roth
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