Thursday, June 18, 2009
Friday, June 12, 2009
Solutions and Formulas
Buffers
0.1M Cacodylate Buffer containing 4% Sucrose and 0.1M Glycine
0.1M Cacodylate Buffer containing 4% sucrose
White's Buffer Solution
0.18M Phosphate Buffer
0.1M Tris Wash
0.4M Phosphate Buffer Stock
0.1M Phosphate Buffer from Monobasic and Dibasic Stock
Fixatives
3% Paraformaldehyde for Immuno Electron Microscopy (Cacodylate Buffer)
Mouse Gluteraldehyde Fixative
Perfusion Fixative in Phosphate Buffer (1%GA, 4%PFA)
2% GA, 2%PFA in 0.1M Phosphate Buffer
2% GA, 2%PFA in 0.1M Cacodylate Buffer
2.5% GA, 4% PFA in 0.1M CB, 0.1M Sucrose
2% GA in 0.05M PB, 0.1M Sucrose
2% OsO4
1% OsO4
Resins
Stains
Urynal Acetate and Lead Citrate for EM section staining
Tuloidine Blue for Semi-Thin OLM Sections
0.1M Cacodylate Buffer containing 4% Sucrose and 0.1M Glycine
0.1M Cacodylate Buffer containing 4% sucrose
White's Buffer Solution
0.18M Phosphate Buffer
0.1M Tris Wash
0.4M Phosphate Buffer Stock
0.1M Phosphate Buffer from Monobasic and Dibasic Stock
Fixatives
3% Paraformaldehyde for Immuno Electron Microscopy (Cacodylate Buffer)
Mouse Gluteraldehyde Fixative
Perfusion Fixative in Phosphate Buffer (1%GA, 4%PFA)
2% GA, 2%PFA in 0.1M Phosphate Buffer
2% GA, 2%PFA in 0.1M Cacodylate Buffer
2.5% GA, 4% PFA in 0.1M CB, 0.1M Sucrose
2% GA in 0.05M PB, 0.1M Sucrose
2% OsO4
1% OsO4
Resins
Stains
Urynal Acetate and Lead Citrate for EM section staining
Tuloidine Blue for Semi-Thin OLM Sections
Thursday, June 11, 2009
Tissue Processing
Click on the tissue that you want to process and all protocols and formulas required will be displayed:
Muscle for Standard TEM
Has been used successfully with with cardiac muscle, diaphragm muscle, leg muscle, and intercostals
Platelets for Standard TEM
Bladder for Immuno Electron Microscopy
Skin
For Skin, we are trying both PB and CB
Drosophila Embryos
Nerve
This protocol has been tested with sciatic nerve, DRG nerve, and optic nerve.
We are trying two different buffers and fixative
Muscle for Standard TEM
Has been used successfully with with cardiac muscle, diaphragm muscle, leg muscle, and intercostals
Platelets for Standard TEM
Bladder for Immuno Electron Microscopy
Skin
For Skin, we are trying both PB and CB
Drosophila Embryos
Nerve
This protocol has been tested with sciatic nerve, DRG nerve, and optic nerve.
We are trying two different buffers and fixative
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