Coming Soon: Catalog numbers by product and company

Protocols

Standard Processing for Immuno Electron Microscopy
Standard Processing for TEM
Processing hard to penetrate samples for TEM
Heavy Metal Staining for TEM Sections

Tools

Making a Grid Transfer Loop

Solutions and Formulas

Buffers
0.1M Cacodylate Buffer containing 4% Sucrose and 0.1M Glycine
0.1M Cacodylate Buffer containing 4% sucrose
White's Buffer Solution
0.18M Phosphate Buffer
0.1M Tris Wash
0.4M Phosphate Buffer Stock
0.1M Phosphate Buffer from Monobasic and Dibasic Stock

Fixatives
3% Paraformaldehyde for Immuno Electron Microscopy (Cacodylate Buffer)
Mouse Gluteraldehyde Fixative
Perfusion Fixative in Phosphate Buffer (1%GA, 4%PFA)
2% GA, 2%PFA in 0.1M Phosphate Buffer
2% GA, 2%PFA in 0.1M Cacodylate Buffer
2.5% GA, 4% PFA in 0.1M CB, 0.1M Sucrose
2% GA in 0.05M PB, 0.1M Sucrose
2% OsO4
1% OsO4

Resins

Stains
Urynal Acetate and Lead Citrate for EM section staining
Tuloidine Blue for Semi-Thin OLM Sections

Tissue Processing

Click on the tissue that you want to process and all protocols and formulas required will be displayed:

Muscle for Standard TEM
Has been used successfully with with cardiac muscle, diaphragm muscle, leg muscle, and intercostals

Platelets for Standard TEM

Bladder for Immuno Electron Microscopy

Skin
For Skin, we are trying both PB and CB

Drosophila Embryos

Nerve
This protocol has been tested with sciatic nerve, DRG nerve, and optic nerve.
We are trying two different buffers and fixative